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Proceedings Paper

Dynamic imaging of transient bubbles generated by femtosecond irradiation of plasmonic nanoparticles in suspensions and cell environment
Author(s): Christos Boutopoulos; Matthieu Fortin-Deschênes; Eric Bergeron; Michel Meunier
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Paper Abstract

Femtosecond (fs) laser generation of submicron bubbles around plasmonic nanoparticles (NPs) plays a key role in advanced laser nanosurgery applications such as cell membrane perforation and cell transfection. In this context, we have developed a pump-probe shadowgraphic ultrafast imaging technique capable of tracking transient bubbles generated by fs irradiation (λ =800 nm, τ = 45 fs) of: (a) plasmonic NPs in suspensions and (b) plasmonic NPs attached to cells. The laser fluence was systematically varied from 100 mJ/cm2 to 500 mJ/cm2 to study the effect on the bubble dynamics generated around 100 nm gold NPs (Au NPs). The plasmonic bubble (PB) generation threshold as well as the NPs deformation threshold were defined. Dark field (DF) imaging and scanning electron microscopy (SEM) revealed NP clustering when 100 nm bare Au NPs were incubated with a cancer cell culture. NP clustering was correlated with PB generation using a combined pump-probe and DF imaging approach. The clustering effect resulted in a significant 4- times decrease to the PB generation threshold compared to single NPs. The clustering effect was further investigated by evaluating bare and polyethylene glycol (PEG) functionalized NPs in terms of PB generation efficiency.

Paper Details

Date Published: 7 March 2014
PDF: 6 pages
Proc. SPIE 8972, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, 897208 (7 March 2014); doi: 10.1117/12.2039951
Show Author Affiliations
Christos Boutopoulos, Ecole Polytechnique de Montréal (Canada)
Matthieu Fortin-Deschênes, Ecole Polytechnique de Montréal (Canada)
Eric Bergeron, Ecole Polytechnique de Montréal (Canada)
Michel Meunier, Ecole Polytechnique de Montréal (Canada)

Published in SPIE Proceedings Vol. 8972:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte, Editor(s)

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