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Proceedings Paper

Hyperspectral (fluorescence lifetime) imaging based on a UV-VIS enhanced supercontinuum source using high-order mode propagation
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Paper Abstract

We report on a new source able to provide probe pulses in the UV visible range and on the demonstration of its application to hyperspectral (fluorescence lifetime) imaging measurements. The source is able to generate UV (down to 300 nm) and blue light exploiting high-order mode propagation in a microstructured fiber pumped by a Ti:Sapphire laser. We believe that further optimization of pump wavelength, fiber length and fiber zero-dispersion wavelength could generate light well below 300 nm using a simple and stable set-up and become a useful tool for biomedical imaging. We demonstrated its versatility using the source for FLIM-FRET measurement a 460 nm and hyperspectral FRET-FLIM measurements.

Paper Details

Date Published: 7 March 2014
PDF: 6 pages
Proc. SPIE 8972, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, 89720M (7 March 2014); doi: 10.1117/12.2039001
Show Author Affiliations
Stefano Taccheo, Swansea Univ. (United Kingdom)
Raffaella Mercatelli, IFAC-CNR (Italy)
Silvia Soria, IFAC-CNR (Italy)
Cosimo D'Andrea, Politecnico di Milano (Italy)
Kay Schuster, Institut für Photonische Technologien e.V. (Germany)
Andreas Bassi, Politecnico di Milano (Italy)
Rinaldo Cubeddu, Politecnico di Milano (Italy)
Jens Kobelke, Institut für Photonische Technologien e.V. (Germany)
Klaus Mörl, Institut für Photonische Technologien e.V. (Germany)
Giancarlo Righini, IFAC-CNR (Italy)
Franco Quercioli, INO-CNR (Italy)


Published in SPIE Proceedings Vol. 8972:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte, Editor(s)

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