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Proceedings Paper

Femtosecond optical injection of intact plant cells using a reconfigurable platform
Author(s): Claire A. Mitchell; Stefan Kalies; Tomas Cizmar; Nicola Bellini; Anisha Kubasik-Thayil; Alexander Heisterkamp; Lesley Torrance; Alison G. Roberts; Frank J. Gunn-Moore; Kishan Dholakia
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Paper Abstract

The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.

Paper Details

Date Published: 7 March 2014
PDF: 6 pages
Proc. SPIE 8972, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, 89720C (7 March 2014); doi: 10.1117/12.2037784
Show Author Affiliations
Claire A. Mitchell, Univ. of St. Andrews (United Kingdom)
Stefan Kalies, Laser Zentrum Hannover e.V. (Germany)
Tomas Cizmar, Univ. of St. Andrews (United Kingdom)
Nicola Bellini, Univ. of St. Andrews (United Kingdom)
Anisha Kubasik-Thayil, Univ. of St. Andrews (United Kingdom)
Alexander Heisterkamp, Friedrich-Schiller-Univ. Jena (Germany)
Lesley Torrance, The James Hutton Institute (United Kingdom)
Univ. of St. Andrews (United Kingdom)
Alison G. Roberts, The James Hutton Institute (United Kingdom)
Frank J. Gunn-Moore, Univ. of St. Andrews (United Kingdom)
Kishan Dholakia, Univ. of St. Andrews (United Kingdom)


Published in SPIE Proceedings Vol. 8972:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte, Editor(s)

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