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Proceedings Paper

Lens-free digital in-line holographic imaging for wide field-of-view, high-resolution and real-time monitoring of complex microscopic objects
Author(s): Richard Stahl; Geert Vanmeerbeeck; Gauthier Lafruit; Roeland Huys; Veerle Reumers; Andy Lambrechts; Chao-Kang Liao; Chin-Chun Hsiao; Masayuki Yashiro; Masashi Takemoto; Tomohisa Nagata; Shinichi Gomi; Kunitada Hatabayashi; Yasuhiro Oshima; Shigenori Ozaki; Naoki Nishishita; Shin Kawamata
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Paper Abstract

Lens-free inline Holographic Microscopy (LHM) holds great promise for biomedical and industrial applications thanks to its conceptual simplicity. However, the challenge lies in achieving an image quality comparable to conventional microscopes. We demonstrate a high-throughput LHM system that is able to resolve 1.23μm-thin lines on a standard USAF 1951 test target with 1.67μm pixels at the full field-of-view (>29mm2). The system is based on a unique multiwavelength iterative-phase-retrieval method, using customized hardware and real-time post-processing software. We have evaluated our system in experiments ranging from single-cell inspection to in-vitro imaging of stem-cell colonies.

Paper Details

Date Published: 4 March 2014
PDF: 10 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471F (4 March 2014); doi: 10.1117/12.2037619
Show Author Affiliations
Richard Stahl, IMEC (Belgium)
Geert Vanmeerbeeck, IMEC (Belgium)
Gauthier Lafruit, IMEC (Belgium)
Roeland Huys, IMEC (Belgium)
Veerle Reumers, IMEC (Belgium)
Andy Lambrechts, IMEC (Belgium)
Chao-Kang Liao, IMEC-Taiwan Co. (Taiwan)
Chin-Chun Hsiao, IMEC-Taiwan Co. (Taiwan)
Masayuki Yashiro, Beatcraft, Inc. (Japan)
Masashi Takemoto, Beatcraft, Inc. (Japan)
Tomohisa Nagata, Tokyo Electron Ltd. (Japan)
Shinichi Gomi, Tokyo Electron Ltd. (Japan)
Kunitada Hatabayashi, Tokyo Electron Ltd. (Japan)
Yasuhiro Oshima, Tokyo Electron Ltd. (Japan)
Shigenori Ozaki, Tokyo Electron Ltd. (Japan)
Naoki Nishishita, Foundation for Biomedical Research and Innovation (Japan)
Shin Kawamata, Foundation for Biomedical Research and Innovation (Japan)

Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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