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Proceedings Paper

Three-dimensional morphological imaging of human induced pluripotent stem cells by using low-coherence quantitative phase microscopy
Author(s): Toyohiko Yamauchi; Yumi Kakuno; Kentaro Goto; Tadashi Fukami; Norikazu Sugiyama; Hidenao Iwai; Yoshinori Mizuguchi; Yutaka Yamashita
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Paper Abstract

There is an increasing need for non-invasive imaging techniques in the field of stem cell research. Label-free techniques are the best choice for assessment of stem cells because the cells remain intact after imaging and can be used for further studies such as differentiation induction. To develop a high-resolution label-free imaging system, we have been working on a low-coherence quantitative phase microscope (LC-QPM). LC-QPM is a Linnik-type interference microscope equipped with nanometer-resolution optical-path-length control and capable of obtaining three-dimensional volumetric images. The lateral and vertical resolutions of our system are respectively 0.5 and 0.93 μm and this performance allows capturing sub-cellular morphological features of live cells without labeling. Utilizing LC-QPM, we reported on three-dimensional imaging of membrane fluctuations, dynamics of filopodia, and motions of intracellular organelles. In this presentation, we report three-dimensional morphological imaging of human induced pluripotent stem cells (hiPS cells). Two groups of monolayer hiPS cell cultures were prepared so that one group was cultured in a suitable culture medium that kept the cells undifferentiated, and the other group was cultured in a medium supplemented with retinoic acid, which forces the stem cells to differentiate. The volumetric images of the 2 groups show distinctive differences, especially in surface roughness. We believe that our LC-QPM system will prove useful in assessing many other stem cell conditions.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471Q (4 March 2014); doi: 10.1117/12.2037106
Show Author Affiliations
Toyohiko Yamauchi, Hamamatsu Photonics K.K. (Japan)
Yumi Kakuno, Hamamatsu Photonics K.K. (Japan)
Kentaro Goto, Hamamatsu Photonics K.K. (Japan)
Tadashi Fukami, Hamamatsu Photonics K.K. (Japan)
Norikazu Sugiyama, Hamamatsu Photonics K.K. (Japan)
Hidenao Iwai, Hamamatsu Photonics K.K. (Japan)
Yoshinori Mizuguchi, Hamamatsu Photonics K.K. (Japan)
Yutaka Yamashita, Hamamatsu Photonics K.K. (Japan)

Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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