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Proceedings Paper

Non-linear imaging and characterization of atherosclerotic arterial tissue using combined two photon fluorescence, second-harmonic generation and CARS microscopy
Author(s): Riccardo Cicchi; Christian Matthäus; Tobias Meyer; Annika Lattermann; Benjamin Dietzek; Bernhard R. Brehm; Jürgen Popp; Francesco S. Pavone
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Paper Abstract

Atherosclerosis is among the most widespread cardiovascular diseases and one of the leading cause of death in the Western World. Characterization of arterial tissue in atherosclerotic condition is extremely interesting from the diagnostic point of view. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires a morpho-functional approach. Multimodal non-linear microscopy has the potential to bridge this gap by providing morpho-functional information on the examined tissues in a label-free way. Here we employed multiple non-linear microscopy techniques, including CARS, TPF, and SHG to provide intrinsic optical contrast from various tissue components in both arterial wall and atherosclerotic plaques. CARS and TPF microscopy were used to respectively image lipid depositions within plaques and elastin in the arterial wall. Cholesterol deposition in the lumen and collagen in the arterial wall were selectively imaged by SHG microscopy and distinguished by forward-backward SHG ratio. Image pattern analysis allowed characterizing collagen organization in different tissue regions. Different values of fiber mean size, distribution and anisotropy are calculated for lumen and media prospectively allowing for automated classification of atherosclerotic lesions. The presented method represents a promising diagnostic tool for evaluating atherosclerotic tissue and has the potential to find a stable place in clinical setting as well as to be applied in vivo in the near future.

Paper Details

Date Published: 28 February 2014
PDF: 6 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894807 (28 February 2014); doi: 10.1117/12.2037018
Show Author Affiliations
Riccardo Cicchi, National Institute of Optics, CNR (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)
Christian Matthäus, Institute of Photonic Technology (Germany)
Tobias Meyer, Institute of Photonic Technology (Germany)
Annika Lattermann, Jena Univ. Hospital, Friedrich-Schiller Univ. (Germany)
Benjamin Dietzek, Institute of Photonic Technology (Germany)
Institute of Physical Chemistry and Abbe Ctr. of Photonics, Friedrich-Schiller Univ. (Germany)
Bernhard R. Brehm, Catholic Clinic, Koblenz (Germany)
Jürgen Popp, Institute of Photonic Technology (Germany)
Institute of Physical Chemistry and Abbe Ctr. of Photonics, Friedrich-Schiller Univ. (Germany)
Francesco S. Pavone, National Institute of Optics, CNR (Italy)
European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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