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Proceedings Paper

Phototransformation of ALA-induced protoporphyrin IX (PPIX) in carcinoma cells and of exogenous PPIX in cells and solutions
Author(s): W. Dietel; Ronald Wendenburg
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Paper Abstract

The fluorescence bleaching and photoproduct formation of PPIX was investigated in Ehrlich ascite carcinoma (EAC) cells (ALA induced and exogenous applied) as well as in micelles (Triton X-100) and DMSO. In all the cases fluorescence bleaching of PPIX occurs but the photoproduct formation is quite different. Besides the rapid fluorescence bleaching of the ALA induced PPIX in EAC cells the formation of a water soluble porphyrin with fluorescence maxima at 616 nm and 678 nm (coproporphyrin) was observed. In the presence of the triplet- and oxygen-quencher (beta) -carotene the fluorescence bleaching of PPIX can be reduced.

Paper Details

Date Published: 1 March 1995
PDF: 5 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203418
Show Author Affiliations
W. Dietel, Jena Univ. (Germany)
Ronald Wendenburg, Jena Univ. (Germany)

Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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