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Proceedings Paper

Tunable laser-based illumination system for cells in monolayer
Author(s): Janet E. Cruse-Sawyer; Mark R. Stringer
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Paper Abstract

A system of testing of potential photosensitizing agents for PDT by the illumination of cells in monolayer is described. The system allows tunability of the excitation wavelength, uniform illumination over a 35 mm cell dish, variation of the irradiance and monitoring of the power delivered throughout the duration of illumination. Knowledge of these parameters allows rapid processing, precise light dosimetry, limitation of possible heating effects as well as optimization of the excitation efficiency. The system is applied to the illumination of an endothelial hybridoma cell line using 680 nm and 692 nm light in the presence of phthalocyanine sensitizers.

Paper Details

Date Published: 1 March 1995
PDF: 3 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203410
Show Author Affiliations
Janet E. Cruse-Sawyer, Univ. of Leeds (United Kingdom)
Mark R. Stringer, Univ. of Leeds (United Kingdom)

Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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