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Proceedings Paper

Surface acoustic streaming in microfluidic system for rapid multicellular tumor spheroids generation
Author(s): Layla AlHasan; Aisha Qi; Aswan Al-Aboodi; Amged Rezk; Richie R. Shilton; Peggy P. Y. Chan; James Friend; Leslie Yeo
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Paper Abstract

In this study, we developed a novel and rapid method to generate in vitro three-dimensional (3D) multicellular tumor spheroids using a surface acoustic wave (SAW) device. A SAW device with single-phase unidirectional transducer electrodes (SPUTD) on lithium niobate substrate was fabricated using standing UV photolithography and wet-etching techniques. To generate spheroids, the SAW device was loaded with medium containing human breast carcinoma (BT474) cells, an oscillating electrical signal at resonant frequency was supplied to the SPUDT to generate acoustic radiation in the medium. Spheroids with uniform size and shape can be obtained using this method in less than 1 minute, and the size of the spheroids can be controlled through adjusting the seeding density. The resulting spheroids were used for further cultivation and were monitored using an optical microscope in real time. The viability and actin organization of the spheroids were assessed using live/dead viability staining and actin cytoskeleton staining, respectively. Compared to spheroids generated using the liquid overlay method, the SAW generated spheroids exhibited higher circularity and higher viability. The F-actin filaments of spheroids appear to aggregate compared to that of untreated cells, indicating that mature spheroids can be obtained using this method. This spheroid generating method can be useful for a variety of biological studies and clinical applications.

Paper Details

Date Published: 7 December 2013
PDF: 4 pages
Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89235C (7 December 2013); doi: 10.1117/12.2034050
Show Author Affiliations
Layla AlHasan, RMIT Univ. (Australia)
Aisha Qi, RMIT Univ. (Australia)
Aswan Al-Aboodi, Monash Univ. (Australia)
Amged Rezk, RMIT Univ. (Australia)
Richie R. Shilton, RMIT Univ. (Australia)
Peggy P. Y. Chan, RMIT Univ. (Australia)
Australian National Fabrication Facility (Australia)
James Friend, RMIT Univ. (Australia)
Australian National Fabrication Facility (Australia)
Leslie Yeo, RMIT Univ. (Australia)

Published in SPIE Proceedings Vol. 8923:
Micro/Nano Materials, Devices, and Systems
James Friend; H. Hoe Tan, Editor(s)

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