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Proceedings Paper

Urothelial conversion of 5-aminolevulinic acid to protoporphyrin IX following oral or intravesical administration
Author(s): Ronald B. Moore; Gerald G. Miller; Kevin Brown; Rakesh Bhatnagar; John Tulip; Malcolm S. McPhee
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Paper Abstract

Preferential conversion of 5-aminolevulinic acid (5-ALA) to protoporphyrin-IX (Pp-IX) occurs in malignant tissue, with accumulation to diagnostic and therapeutic levels. Recent studies have suggested selective conversion in epithelial tissue following oral or intravenous administration. Topical application avoids systemic photosensitization. However, the glycosaminoglycan (GAG) layer lining the urinary bladder is believed to be a protective barrier generally limiting mucosal absorption. Our objective was to evaluate uptake and conversion of 5-ALA following intravesical or oral administration. Using a rat model, Pp-IX content within epithelial and muscularis layers was quantitated by fluorescence confocal microscopy. Following intravesical administration, Pp-IX accumulated predominantly in the urothelium; whereas following oral administration, Pp-IX accumulated in both the urothelium and muscularis. Intravesical 5-ALA administration is feasible and may afford selective photosensitization of the urothelium for treatment of carcinoma in situ.

Paper Details

Date Published: 1 March 1995
PDF: 5 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203359
Show Author Affiliations
Ronald B. Moore, Cross Cancer Institute (Canada)
Gerald G. Miller, Cross Cancer Institute (Canada)
Kevin Brown, Cross Cancer Institute (Canada)
Rakesh Bhatnagar, Univ. of Alberta (Canada)
John Tulip, Univ. of Alberta (Canada)
Malcolm S. McPhee, Cross Cancer Institute (Canada)

Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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