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Proceedings Paper

Detection of singlet oxygen yield from new photosensitizers using luminol
Author(s): Harumasa Sakai; Hugo Oppelaar; Paul Baas; Nico Van Zandwijk; Fiona A. Stewart
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Paper Abstract

For the application of photodynamic therapy and diagnosis many different photosensitizers have been developed. It is important to compare these photosensitizers for their activity. It is generally accepted that the most important mechanism of cell killing is via the production of singlet oxygen. We therefore performed basic studies to detect singlet oxygen using a luminol reaction. The relative singlet oxygen yields from 4 photosensitizers (Photofrin, ATX-S10, mTHPC and NPe6) were measured by the detection of luminol chemiluminescence at 445 nm wavelength in Menzel's buffer solution at pH 10.5. NPe6, ATX-S10 and mTHPC all showed singlet oxygen productive abilities. These photosensitizers are new promising photosensitizers. These results show a possibility of comparison of each photosensitizer.

Paper Details

Date Published: 1 March 1995
PDF: 4 pages
Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); doi: 10.1117/12.203354
Show Author Affiliations
Harumasa Sakai, European Cancer Ctr. (Netherlands)
Hugo Oppelaar, The Netherlands Cancer Institute (Netherlands)
Paul Baas, The Netherlands Cancer Institute (Netherlands)
Nico Van Zandwijk, The Netherlands Cancer Institute (Netherlands)
Fiona A. Stewart, The Netherlands Cancer Institute (Netherlands)

Published in SPIE Proceedings Vol. 2371:
5th International Photodynamic Association Biennial Meeting
Denis A. Cortese, Editor(s)

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