In this paper the dimerization constant of pure hematoporphyrine was established using absorption method and then fluorescence quantum yield ((phi) ) was calculated as a function of monomeric fraction ((alpha) ). It was our objective to formulate an equation describing the relationships between (phi) , (alpha) and ratio of monomer to dimer molar absorption coefficients ((epsilon) _M/(epsilon) _D), at a given wavelength. (phi) was also studied as a function of pH value and polarity. A 3-D stack fluorescence plot was also constructed and the possibilities of practical application of the fluorescence effect to the visualization of areas with absorbed are presented.

Date Published: 1 March 1995
PDF: 7 pages
Proc. SPIE 2203, Laser Technology IV: Applications in Medicine, (1 March 1995); doi: 10.1117/12.203332

Published in SPIE Proceedings Vol. 2203:
Laser Technology IV: Applications in Medicine
Wieslaw L. Wolinski; Tadeusz Kecik, Editor(s)