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Proceedings Paper

Cardiac action potential imaging
Author(s): Qinghai Tian; Peter Lipp; Lars Kaestner
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Paper Abstract

Action potentials in cardiac myocytes have durations in the order of magnitude of 100 milliseconds. In biomedical investigations the documentation of the occurrence of action potentials is often not sufficient, but a recording of the shape of an action potential allows a functional estimation of several molecular players. Therefore a temporal resolution of around 500 images per second is compulsory. In the past such measurements have been performed with photometric approaches limiting the measurement to one cell at a time. In contrast, imaging allows reading out several cells at a time with additional spatial information. Recent developments in camera technologies allow the acquisition with the required speed and sensitivity. We performed action potential imaging on isolated adult cardiomyocytes of guinea pigs utilizing the fluorescent membrane potential sensor di-8-ANEPPS and latest electron-multiplication CCD as well as scientific CMOS cameras of several manufacturers. Furthermore, we characterized the signal to noise ratio of action potential signals of varying sets of cameras, dye concentrations and objective lenses. We ensured that di-8-ANEPPS itself did not alter action potentials by avoiding concentrations above 5 μM. Based on these results we can conclude that imaging is a reliable method to read out action potentials. Compared to conventional current-clamp experiments, this optical approach allows a much higher throughput and due to its contact free concept leaving the cell to a much higher degree undisturbed. Action potential imaging based on isolated adult cardiomyocytes can be utilized in pharmacological cardiac safety screens bearing numerous advantages over approaches based on heterologous expression of hERG channels in cell lines.

Paper Details

Date Published: 18 June 2013
PDF: 7 pages
Proc. SPIE 8798, Clinical and Biomedical Spectroscopy and Imaging III, 879807 (18 June 2013); doi: 10.1117/12.2032564
Show Author Affiliations
Qinghai Tian, Saarland Univ. (Germany)
Peter Lipp, Saarland Univ. (Germany)
Lars Kaestner, Saarland Univ. (Germany)

Published in SPIE Proceedings Vol. 8798:
Clinical and Biomedical Spectroscopy and Imaging III
Volker Deckert; Nirmala Ramanujam, Editor(s)

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