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Proceedings Paper

Spatial light modulation for improved microscope stereo vision and 3D tracking
Author(s): Michael P. Lee; Graham Gibson; Manlio Tassieri; Dave Phillips; Stefan Bernet; Monika Ritsh-Marte; Miles J. Padgett
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Paper Abstract

We present a new type of stereo microscopy which can be used for tracking in 3D over an extended depth. The use of Spatial Light Modulators (SLMs) in the Fourier plane of a microscope sample is a common technique in Holographic Optical Tweezers (HOT). This set up is readily transferable from a tweezer system to an imaging system, where the tweezing laser is replaced with a camera. Just as a HOT system can diffract many traps of different types, in the imaging system many different imaging types can be diffracted with the SLM. The type of imaging we have developed is stereo imaging combined with lens correction. This approach has similarities with human vision where each eye has a lens, and it also extends the depth over which we can accurately track particles.

Paper Details

Date Published: 12 September 2013
PDF: 8 pages
Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 881022 (12 September 2013); doi: 10.1117/12.2027734
Show Author Affiliations
Michael P. Lee, Univ. of Glasgow (United Kingdom)
Graham Gibson, Univ. of Glasgow (United Kingdom)
Manlio Tassieri, Univ. of Glasgow (United Kingdom)
Dave Phillips, Univ. of Bristol (United Kingdom)
Stefan Bernet, Innsbruck Medical Univ. (Austria)
Monika Ritsh-Marte, Innsbruck Medical Univ. (Austria)
Miles J. Padgett, Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 8810:
Optical Trapping and Optical Micromanipulation X
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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