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Proceedings Paper

Evidence for non-equilibrium dynamics in viral DNA packaging from optical tweezers measurements
Author(s): Zachary T. Berndsen; Nicholas Keller; Douglas E. Smith
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Paper Abstract

In many viruses molecular motors generate large forces to package DNA to high densities. The dynamics and energetics of this process is a subject of wide debate and is of interest as a model for studying confined polymer physics in general. Here we present preliminary results showing that DNA in bacteriophage phi29 undergoes nonequilibrium conformational dynamics during packaging with a relaxation time >60,000x longer than for free DNA and >3000x longer than reported for DNA confined in nanochannels. Nonequilibrium dynamics significantly increases the load on the motor, causes heterogeneity in the rates of packaging, and causes frequent pausing in motor translocation.

Paper Details

Date Published: 12 September 2013
PDF: 10 pages
Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 881033 (12 September 2013); doi: 10.1117/12.2027187
Show Author Affiliations
Zachary T. Berndsen, Univ. of California, San Diego (United States)
Nicholas Keller, Univ. of California, San Diego (United States)
Douglas E. Smith, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 8810:
Optical Trapping and Optical Micromanipulation X
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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