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Proceedings Paper

Evaluating cell matrix mechanics using an integrated nonlinear optical tweezer-confocal imaging system
Author(s): Berney Peng; Carlo A. C. Alonzo; Lawrence Xia; Lucia Speroni; Irene Georgakoudi; Ana M. Soto; Carlos Sonnenschein; Mark Cronin-Golomb
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Paper Abstract

Biomechanics plays a central role in breast epithelial morphogenesis. In this study we have used 3D cultures in which normal breast epithelial cells are able to organize into rounded acini and tubular ducts, the main structures found in the breast tissue. We have identified fiber organization as a main determinant of ductal organization. While bulk rheological properties of the matrix seem to play a negligible role in determining the proportion of acini versus ducts, local changes may be pivotal in shape determination. As such, the ability to make microscale rheology measurements coupled with simultaneous optical imaging in 3D cultures can be critical to assess the biomechanical factors underlying epithelial morphogenesis. This paper describes the inclusion of optical tweezers based microrheology in a microscope that had been designed for nonlinear optical imaging of collagen networks in ECM. We propose two microrheology methods and show preliminary results using a gelatin hydrogel and collagen/Matrigel 3D cultures containing mammary gland epithelial cells.

Paper Details

Date Published: 12 September 2013
PDF: 8 pages
Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88101O (12 September 2013); doi: 10.1117/12.2027155
Show Author Affiliations
Berney Peng, Tufts Univ. (United States)
Carlo A. C. Alonzo, Tufts Univ. (United States)
Lawrence Xia, Tufts Univ. (United States)
Lucia Speroni, Tufts Univ. School of Medicine (United States)
Irene Georgakoudi, Tufts Univ. (United States)
Ana M. Soto, Tufts Univ. School of Medicine (United States)
Carlos Sonnenschein, Tufts Univ. School of Medicine (United States)
Mark Cronin-Golomb, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 8810:
Optical Trapping and Optical Micromanipulation X
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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