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Proceedings Paper

An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets
Author(s): Alexander L. Hargreaves; Andrew K. Kirby; Colin D. Bain; Gordon D. Love; Guido Bolognesi; Oscar Ces; Mark Neil; Andrew D. Ward
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Paper Abstract

We discuss the design, implementation and performance of a novel platform for the production and optical control of ultra-low interfacial tension droplets in the 1-10 micron regime. A custom-designed, integrated microfluidic system allows the production of oil-in-water emulsion droplets of controllable size. This provides an optimised physical platform in which individual droplets are selected, trapped and shaped by holographic optical tweezers (HOTs) via extended optical landscaping. The 3D structure of the shaped droplet is interrogated by a combination of conventional brightfield imaging and fluorescent structured-illumination sectioning. We detail the problems and limitations of closed-loop holographic control of droplet shape.

Paper Details

Date Published: 12 September 2013
PDF: 13 pages
Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88101W (12 September 2013); doi: 10.1117/12.2027105
Show Author Affiliations
Alexander L. Hargreaves, Univ. of Durham (United Kingdom)
Andrew K. Kirby, Univ. of Durham (United Kingdom)
Colin D. Bain, Univ. of Durham (United Kingdom)
Gordon D. Love, Univ. of Durham (United Kingdom)
Guido Bolognesi, Imperial College London (United Kingdom)
Oscar Ces, Imperial College London (United Kingdom)
Mark Neil, Imperial College London (United Kingdom)
Andrew D. Ward, Rutherford Appleton Lab. (United Kingdom)

Published in SPIE Proceedings Vol. 8810:
Optical Trapping and Optical Micromanipulation X
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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