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Proceedings Paper

Diffuse reflectance spectroscopy and optical polarization imaging of in-vivo biological tissue
Author(s): A. Mora-Núñez; Y. Castillejos; G. García-Torales; G. Martínez-Ponce
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Paper Abstract

A number of optical techniques have been reported in the scientific literature as accomplishable methodologies to diagnose diseases in biological tissue, for instance, diffuse reflectance spectroscopy (DRS) and optical polarization imaging (OPI). The skin is the largest organ in the body and consists of three primary layers, namely, the epidermis (the outermost layer exposed to the world), the dermis, and the hypodermis. The epidermis changes from to site to site, mainly because of difference in hydration. A lower water content increase light scattering and reduce the penetration depth of radiation. In this work, two hairless mice have been selected to evaluate their skin features by using DRS and OPI. Four areas of the specimen body were chosen to realize the comparison: back, abdomen, tail, and head. From DRS, it was possible to distinguish the skin nature because of different blood irrigation at dermis. In the other hand, OPI shows pseudo-depolarizing regions in the measured Mueller images related to a spatially varying propagation of the scattered light. This provides information about the cell size in the irradiated skin.

Paper Details

Date Published: 18 November 2013
PDF: 7 pages
Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785D7 (18 November 2013); doi: 10.1117/12.2025878
Show Author Affiliations
A. Mora-Núñez, Univ. de Guadalajara (Mexico)
Y. Castillejos, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
G. García-Torales, Univ. de Guadalajara (Mexico)
G. Martínez-Ponce, Ctr. de Investigaciones en Óptica, A.C. (Mexico)


Published in SPIE Proceedings Vol. 8785:
8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications
Manuel Filipe P. C. Martins Costa, Editor(s)

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