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Proceedings Paper

A long period grating-based platform for the detection of E. coli proteins
Author(s): R. B. Queirós; C. Gouveia; J. R. A. Fernandes; P. V. S. Marques; J. P. Noronha; M. G. F. Sales; P. A. S. Jorge
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Paper Abstract

A Long Period Grating (LPG)-based platform for the detection of E. coli outer membranes proteins (EcOMPs) is presented. The sensing probe is achieved by the functionalization of a LPG inscribed in a single mode fiber (SMF28) with a DNA-aptamer resulting in a label-free configuration capable of specific recognize EcOMPs in waters. Measurement takes place by tracking the variations induced in the resonance wavelength by the refractive index changes at the fiber surface (≈100 nm/RIU). The sensing head was characterized and tested against EcOMPs and applied to spiked environmental water samples. The sensor displayed logarithmic responses in the range of 0.1 nM to 10 nM EcOMPs and is regenerated (under low pH conditions) and the deviation of the subsequent detection was less than 0.1 %.

Paper Details

Date Published: 18 November 2013
PDF: 6 pages
Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785EY (18 November 2013); doi: 10.1117/12.2025811
Show Author Affiliations
R. B. Queirós, INESC Porto (Portugal)
Univ. do Porto (Portugal)
Instituto Politécnico do Porto (Portugal)
C. Gouveia, INESC Porto (Portugal)
J. R. A. Fernandes, Univ. de Trás-os-Montes e Alto Douro (Portugal)
P. V. S. Marques, INESC Porto (Portugal)
Univ. do Porto (Portugal)
J. P. Noronha, Univ. Nova de Lisboa (Portugal)
M. G. F. Sales, Instituto Politécnico do Porto (Portugal)
P. A. S. Jorge, INESC Porto (Portugal)


Published in SPIE Proceedings Vol. 8785:
8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications
Manuel Filipe P. C. Martins Costa, Editor(s)

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