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Proceedings Paper

Rheology at the micro-scale: new tools for bio-analysis
Author(s): Rebecca L. Warren; Manlio Tassieri; Xiang Li; Andrew Glidle; David J. Paterson; Allan Carlsson; Jonathan M. Cooper
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Paper Abstract

We present a simple and non-invasive experimental procedure to measure the linear viscoelastic properties of cells by passive particle tracking microrheology. In order to do this, a generalised Langevin equation is adopted to relate the timedependent thermal fluctuations of a probe sensor, immobilised to the cell’s membrane, to the frequency-dependent viscoelastic moduli of the cell. The method has been validated by measuring the linear viscoelastic response of a soft solid and then applied to cell physiology studies. It is shown that the viscoelastic moduli are related to the cell’s cytoskeletal structure, which in this work is modulated either by inhibiting the actin/myosin-II interactions by means of blebbistatin or by varying the solution osmolarity from iso- to hypo-osmotic conditions. The insights gained from this form of rheological analysis promises to be a valuable addition to physiological studies; e.g. cell physiology during pathology and pharmacological response.

Paper Details

Date Published: 23 May 2013
PDF: 13 pages
Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 879214 (23 May 2013); doi: 10.1117/12.2025254
Show Author Affiliations
Rebecca L. Warren, Univ. of Glasgow (United Kingdom)
Manlio Tassieri, Univ. of Glasgow (United Kingdom)
Xiang Li, Univ. of Glasgow (United Kingdom)
Andrew Glidle, Univ. of Glasgow (United Kingdom)
David J. Paterson, Univ. of Glasgow (United Kingdom)
Allan Carlsson, Univ. of Glasgow (United Kingdom)
Jonathan M. Cooper, Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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