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Proceedings Paper

Detection of saccharides with a fluorescent sensing device based on a gold film modified with 4-mercaptophenylboronic acid monolayer
Author(s): Shu-Jen Chen; Jui-Feng Chang; Nai-Jen Cheng; Jeng-Nan Yih; Kuo-Chi Chiu
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Paper Abstract

An extremely sensitive fluorescent sensor based on a phenylboronic acid monolayer was developed for detecting saccharide molecules. The fluorescent sensor was prepared by assembling a monolayer of 4-mercaptophenylboronic acid (4-MPBA) onto a gold-coated compact disk. The change in the fluorescence of the 4-MPBA monolayer was extremely obvious in basic methanolic buffer containing monosaccharides down to the picomolar level. The fluorescence spectra demonstrated that the 4-MPBA monolayer was sensitive to monosaccharides and disaccharides, and the affinity of the monolayer toward saccharides was in the order of glucose < fructose < mannose < galactose < maltose > lactose > sucrose. Additionally, the fluorescence intensity of 4-MPBA monolayer was restorable after cleaning with weak acid, indicating that the reported fluorescent sensor with the detection limit of glucose down to the picomolar level is reusable for sensing saccharides.

Paper Details

Date Published: 11 September 2013
PDF: 9 pages
Proc. SPIE 8812, Biosensing and Nanomedicine VI, 881210 (11 September 2013); doi: 10.1117/12.2023060
Show Author Affiliations
Shu-Jen Chen, National Kaohsiung Univ. of Applied Sciences (Taiwan)
Jui-Feng Chang, National Kaohsiung Univ. of Applied Sciences (Taiwan)
Nai-Jen Cheng, National Kaohsiung Univ. of Applied Sciences (Taiwan)
Jeng-Nan Yih, National Kaohsiung Univ. of Applied Sciences (Taiwan)
Kuo-Chi Chiu, Industrial Technology Research Institute (Taiwan)

Published in SPIE Proceedings Vol. 8812:
Biosensing and Nanomedicine VI
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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