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Low power laser and LED irradiation effect on proliferation and differentiation of Wistar rats mesenchymal stem cells
Author(s): Diana Mancera; Efrain Solarte; Leonardo Fierro; William Criollo
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Paper Abstract

It has been demonstrated that appropriately cultured and stimulated mesenchymal cells, can give rise to cells of all tissues of the body. We evaluate the cell proliferation and differentiation induced by low power light irradiation in cell cultures of mesenchymal cells, isolated and previously characterized, from Wistar rats. Roche® XTT and LDH tests were used to assess proliferation and cytotoxicity. Cellular differentiation was determined by optical microscopy and using specific fluorescent markers. We report laser cellular proliferation enhancement by 532 and 473 nm, and the best cell culture response by a dose of 2 Jcm-2. Although a three day irradiation protocol the cultures grown and no cytotoxicity was detected. Cellular differentiation occurred, and the production of cardiomyocytes was promoted by the cell proliferation stimulated by low power laser irradiation.

Paper Details

Date Published: 18 November 2013
PDF: 9 pages
Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785DY (18 November 2013); doi: 10.1117/12.2021766
Show Author Affiliations
Diana Mancera, Univ. del Valle (Colombia)
Efrain Solarte, Univ. del Valle (Colombia)
Leonardo Fierro, Univ. del Valle (Colombia)
William Criollo, Univ. del Valle (Colombia)

Published in SPIE Proceedings Vol. 8785:
8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications
Manuel Filipe P. C. Martins Costa, Editor(s)

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