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Proceedings Paper

Investigation on 3D morphological changes of in vitro cells through digital holographic microscopy
Author(s): Pasquale Memmolo; Lisa Miccio; Francesco Merola; Paolo A. Netti; Giuseppe Coppola; Pietro Ferraro
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Paper Abstract

We report the investigation of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps (QPMs) of biological cells by digital holographic microscopy (DHM), with the aim to analyze the 3D positions and 3D morphology together. We consider as test case for our tool the in vitro bull sperm head morphometry analysis. Extraction and measurement of various morphological parameters are performed by using two methods: the anisotropic diffusion filter, that is based on the Gaussian diffusivity function which allows more accuracy of the edge position, and the simple thresholding filter. In particular we consider the calculation of area, ellipticity, perimeter, major axis, minor axis and shape factor as a morphological parameter, instead, for the estimation of 3D position, we compute the centroid, the weighted centroid and the maximum phase values. A statistical analysis on a data set composed by N = 14 holograms relative to bovine spermatozoa and its reference holograms is reported.

Paper Details

Date Published: 23 May 2013
PDF: 6 pages
Proc. SPIE 8792, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, 87920R (23 May 2013); doi: 10.1117/12.2020570
Show Author Affiliations
Pasquale Memmolo, Istituto Italiano di Tecnologia (Italy)
Istituto Nazionale di Ottica, CNR (Italy)
Lisa Miccio, Istituto Nazionale di Ottica, CNR (Italy)
Francesco Merola, Istituto Nazionale di Ottica, CNR (Italy)
Paolo A. Netti, Istituto Italiano di Tecnologia (Italy)
Giuseppe Coppola, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
Pietro Ferraro, Istituto Nazionale di Ottica, CNR (Italy)

Published in SPIE Proceedings Vol. 8792:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials
Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, Editor(s)

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