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Proceedings Paper

Deconvolution microscopy of living cells for phase-contrast imaging
Author(s): Guanxiao Cheng; Ping Xu; Zhilong Sun; Chunquan Hong; Zelin Li
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Paper Abstract

The goal of deconvolution microscopy for phase-contrast imaging is to reassign the optical blur to its original position and to reduce statistical noise, thus visualizing the cellular structures of living cells in three dimensions and at subresolution scale. The major features of this technology for a phase-contrast microscopy are discussed through a series of theoretical analyses. A few of possible sources of aberrations and image degradation processes are presented. The theoretical and experimental results have shown that deconvolution microscopy can enhance resolution and contrast by either subtracting or reassigning out-of-focus blur.

Paper Details

Date Published: 13 May 2013
PDF: 6 pages
Proc. SPIE 8789, Modeling Aspects in Optical Metrology IV, 87890F (13 May 2013); doi: 10.1117/12.2020372
Show Author Affiliations
Guanxiao Cheng, Shenzhen Univ. (China)
Ping Xu, Shenzhen Univ. (China)
Zhilong Sun, Shenzhen Univ. (China)
Chunquan Hong, Shenzhen Univ. (China)
Zelin Li, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 8789:
Modeling Aspects in Optical Metrology IV
Bernd Bodermann; Karsten Frenner; Richard M. Silver, Editor(s)

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