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Proceedings Paper

DNA biosensors implemented on PNA-functionalized microstructured optical fibers Bragg gratings
Author(s): A. Candiani; S. Giannetti; A. Cucinotta; A. Bertucci; A. Manicardi; M. Konstantaki; W. Margulis; S. Pissadakis; R. Corradini; S. Selleri
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Paper Abstract

A novel DNA sensing platform based on a Peptide Nucleic Acid - functionalized Microstructured Optical Fibers gratings has been demonstrated. The inner surface of different MOFs has been functionalized using PNA probes, OligoNucleotides mimic that are well suited for specific DNA target sequences detection. The hybrid sensing systems were tested for optical DNA detection of targets of relevance in biomedical application, using the cystic fibrosis gene mutation, and food-analysis, using the genomic DNA from genetic modified organism soy flour. After the solutions of DNA molecules has been infiltrated inside the fibers capillaries and hybridization has occurred, oligonucleotidefunctionalized gold nanoparticles were infiltrated and used to form a sandwich-like system to achieve signal amplification. Spectral measurements of the reflected signal reveal a clear wavelength shift of the reflected modes when the infiltrated complementary DNA matches with the PNA probes placed on the inner fiber surface. Measurements have also been made using the mismatched DNA solution for the c, containing a single nucleotide polymorphism, showing no significant changes in the reflected spectrum. Several experiments have been carried out demonstrating the reproducibility of the results and the high selectivity of the sensors, showing the simplicity and the potential of this approach.

Paper Details

Date Published: 3 May 2013
PDF: 7 pages
Proc. SPIE 8775, Micro-structured and Specialty Optical Fibres II, 877502 (3 May 2013); doi: 10.1117/12.2016684
Show Author Affiliations
A. Candiani, Univ. degli Studi di Parma (Italy)
S. Giannetti, Univ. degli Studi di Parma (Italy)
A. Cucinotta, Univ. degli Studi di Parma (Italy)
A. Bertucci, Univ. degli Studi di Parma (Italy)
A. Manicardi, Univ. degli Studi di Parma (Italy)
M. Konstantaki, Foundation for Research and Technology-Hellas (Greece)
W. Margulis, Acreo Swedish ICT AB (Sweden)
S. Pissadakis, Foundation for Research and Technology-Hellas (Greece)
R. Corradini, Univ. degli Studi di Parma (Italy)
S. Selleri, Univ. degli Studi di Parma (Italy)

Published in SPIE Proceedings Vol. 8775:
Micro-structured and Specialty Optical Fibres II
Kyriacos Kalli; Jiri Kanka; Alexis Mendez, Editor(s)

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