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Proceedings Paper

Developing a cell-based sensor for the detection of Autoinducer-2
Author(s): Matthew D. Servinsky; Katherine Germane; Elliot S. Gerlach; Chen-Yu Tsao; Christopher M. Byrd; Christian J. Sund; William E. Bentley
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Paper Abstract

Bacteria use an intricate set of communication systems for sensing and interpreting environmental cues that coordinate population-based behavior. Quorum sensing is one of these systems, and it involves the production, release, and detection of small chemical signaling molecules. Recent research has revealed the role of quorum sensing molecules in the control of microbial activities such as biofilm formation. In this presentation we outline the development of a recombinant E. coli cell-based sensor for detection of the quorum sensing molecule Autoinducer-2 (AI-2), as well as engineering strategies to remove sugar and anoxic inhibition of the strain.

Paper Details

Date Published: 5 June 2013
PDF: 8 pages
Proc. SPIE 8719, Smart Biomedical and Physiological Sensor Technology X, 871903 (5 June 2013); doi: 10.1117/12.2016601
Show Author Affiliations
Matthew D. Servinsky, U.S. Army Research Lab. (United States)
Katherine Germane, U.S. Army Research Lab. (United States)
Elliot S. Gerlach, U.S. Army Research Lab. (United States)
Chen-Yu Tsao, U.S. Army Research Lab. (United States)
Christopher M. Byrd, Univ. of Maryland (United States)
Christian J. Sund, U.S. Army Research Lab. (United States)
William E. Bentley, Univ. of Maryland (United States)

Published in SPIE Proceedings Vol. 8719:
Smart Biomedical and Physiological Sensor Technology X
Brian M. Cullum; Eric S. McLamore, Editor(s)

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