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Proceedings Paper

Surface plasmon resonance response of a polymer-coated biochemical sensor
Author(s): Marie-Claude Millot; Thierry Vals; Francoise Martin; Bernard Sebille; Yves Levy
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Paper Abstract

Surface plasmon resonance is an optical phenomenon which can be used to monitor the increase in the thickness of a dielectric layer, as an antigen binds to an immobilized antibody. In the present work, the preparation of a reactive surface which permits covalent immobilization of proteins and antigen-antibody reactions is described. In a first step, cysteamine a bifunctional reagent, is linked to a thin gold film via thiol groups. A reactive polymer is then covalently bound to the cysteamine layer. The resulting surface permits immobilization of proteins through their amino groups. The polymer layer is hydrophilic and not ionic in order to prevent hydrophobic and/or electrostatic interactions with proteins. After deactivation of the residual reactive functions, the binding of rabbit immunoglobulin G (IgG) onto this surface, rabbit IgG-anti rabbit IgG interactions and specificity of the system were studied. Preliminary results concerning stability and regeneration of the biosensor are reported.

Paper Details

Date Published: 10 February 1995
PDF: 6 pages
Proc. SPIE 2331, Medical Sensors II and Fiber Optic Sensors, (10 February 1995); doi: 10.1117/12.201249
Show Author Affiliations
Marie-Claude Millot, Univ. Paris XII/CNRS (France)
Thierry Vals, Univ. Paris XII/CNRS (France)
Francoise Martin, Institut d'Optique/CNRS (France)
Bernard Sebille, Univ. Paris XII/CNRS (France)
Yves Levy, Institut d'Optique/CNRS (France)

Published in SPIE Proceedings Vol. 2331:
Medical Sensors II and Fiber Optic Sensors
Anna Maria Verga Scheggi; Francesco Baldini; Pierre R. Coulet; Otto S. Wolfbeis, Editor(s)

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