
Proceedings Paper
Microscopic imaging of glyceraldehyde-induced tissue glycation with intrinsic second harmonic generation and two-photon fluorescence contrastsFormat | Member Price | Non-Member Price |
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Paper Abstract
The bioinspired approaches to tissue strengthening and preservation rely on non-toxic
cross-linking agents one of which is glyceraldehyde. In this study we used multiphoton
microscopy that employs second harmonic generation (SHG) contrast to evaluate
collagen microstructures and two-photon fluorescence (TPF) contrast to monitor
progression of cross-linking upon treatment of tissues with glyceraldehyde. We examined
collagen hydrogels assembled at 37 °C and 27 °C, bovine scleral and corneal tissues, skin
as well as rat tail tendons. The results show a different effect of glyceraldehyde on
collagen microstructures within the above tissues. This effect depends on the original
microstructural assembly of collagen within a specific tissue. Our data suggests that
epidermis (in skin and cornea) will protect collagen from cross-linking with
glyceraldehyde. The work highlights benefits of monitoring progression of collagen
cross-linking and effects of cross-linking on fiber microstructures as imaged with SHG
and TPF signals.
Paper Details
Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858725 (22 February 2013); doi: 10.1117/12.2012305
Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
PDF: 7 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858725 (22 February 2013); doi: 10.1117/12.2012305
Show Author Affiliations
Yu Jer Hwang, Univ. of California, Riverside (United States)
Joseph Granelli, Univ. of California, Riverside (United States)
Joseph Granelli, Univ. of California, Riverside (United States)
Manasa Tirumalasetty, Univ. of California, Riverside (United States)
Julia Lyubovitsky, Univ. of California, Riverside (United States)
Julia Lyubovitsky, Univ. of California, Riverside (United States)
Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
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