The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor.

Date Published: 18 December 2012
PDF: 6 pages
Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970Y (18 December 2012); doi: 10.1117/12.2010539

Published in SPIE Proceedings Vol. 8697:
18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Jan Peřina Jr.; Libor Nozka; Miroslav Hrabovský; Dagmar Senderáková; Waclaw Urbańczyk; Ondrej Haderka; Libor Nožka, Editor(s)