
Proceedings Paper
Evaluating cerebellar functions using optogenetic transgenic miceFormat | Member Price | Non-Member Price |
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Paper Abstract
We employed a transgenic mouse having conditional expression of ChR2(H134R) in neurons of the inferior olive to
facilitate understanding of the role of electrical coupling and oscillation in central nervous system function. Two-photon
excitation of ChR2-expressing neurons using 64 laser beams restricted to single inferior olive cell bodies depolarized
neurons and evoked voltage deflections in neighboring neurons demonstrating electrical coupling. Broader illumination
of neuronal ensembles using blue light induced an optical clamp of endogenous electrical rhythms in the inferior olive of
acutely-prepared brain slices, which when applied in vivo directly modulated the local field potential activity and
induced tremor. The experiments demonstrate novel methods to optically manipulate electrically coupled potentials and
rhythmogenesis within a neuronal ensemble. From a functional perspective, the experiments shed light on the cellular
and circuitry mechanisms of essential tremor, a prevalent neurological condition, by indicating time- and frequencydependence
of tremor upon varying rhythms of inferior olive stimulation. The experiments indicate analog control of a
brain rhythm that may be used to enhance our understanding of the functional consequences of central rhythmogenesis.
Paper Details
Date Published: 8 March 2013
PDF: 9 pages
Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860C (8 March 2013); doi: 10.1117/12.2010204
Published in SPIE Proceedings Vol. 8586:
Optogenetics: Optical Methods for Cellular Control
Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)
PDF: 9 pages
Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860C (8 March 2013); doi: 10.1117/12.2010204
Show Author Affiliations
John P. Welsh, Seattle Children's Research Institute (United States)
Univ. of Washington (United States)
Josef Turecek, Seattle Children's Research Institute (United States)
Univ. of Washington (United States)
Josef Turecek, Seattle Children's Research Institute (United States)
Eric E. Turner, Seattle Children's Research Institute (United States)
Univ. of Washington (United States)
Univ. of Washington (United States)
Published in SPIE Proceedings Vol. 8586:
Optogenetics: Optical Methods for Cellular Control
Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)
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