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Proceedings Paper

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission
Author(s): Jianhong Ge; Yixin Wang; Cuifang Kuang; Shin-Shian Lee; Nirmal Mazumder; Fu-Jen Kao
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Paper Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858832 (22 February 2013); doi: 10.1117/12.2009387
Show Author Affiliations
Jianhong Ge, Zhejiang Univ. (China)
Yixin Wang, Zhejiang Univ. (China)
Cuifang Kuang, Zhejiang Univ. (China)
Shin-Shian Lee, National Yang-Ming Univ. (Taiwan)
Nirmal Mazumder, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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