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Proceedings Paper

Changes in the light path length of blood-perfused rat liver by increased hematocrit and anoxia
Author(s): Toshiyuki Kitai; Bertrand Beauvoit; Hanli Liu; Britton Chance
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Paper Abstract

The purpose of this study is to evaluate the usefulness of time-resolved spectroscopy (TRS) and phase modulation spectroscopy (PMS) for the measurement of hemoglobin saturation (SO2) in the liver. Our materials and methods were: (1) Absorption coefficient ((mu) a) and reduced scattering coefficient (microsecond(s) ') of in situ rat liver, blood-free rat liver and red blood cell (RBC) suspension were measured by TRS. (2) Changes in the light path length of blood-perfused rat liver by increasing hematocrit (3%, 12%, and 36%) and anoxia were measured by PMS. Our results show: (1) (mu) a of in situ rat liver could not be determined, because the absorption was too high. From (mu) a and microsecond(s) ' values of blood-free liver and RBC suspension, (mu) a and microsecond(s) ' of normal-hematocrit liver was extrapolated as 1.08 cm-1 and 15.46 cm-1 at 780 nm, respectively. (2) Although the trend of liver SO2 by increasing hematocrit was reasonable, there was a discrepancy between liver SO2 and output SO2 by anoxia. Changes in the light path length of the liver were as small as 10% of the total light path length. We conclude: Quantitation of liver SO2 by TRS and PMS was difficult because of its very high (mu) a. Since changes in the light path length were small, continuous wave spectroscopy would be an effective way to monitor the oxygenation state of the liver.

Paper Details

Date Published: 31 January 1995
PDF: 8 pages
Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); doi: 10.1117/12.200859
Show Author Affiliations
Toshiyuki Kitai, Univ. of Pennsylvania (United States)
Bertrand Beauvoit, Univ. of Pennsylvania (United States)
Hanli Liu, Univ. of Pennsylvania (United States)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 2326:
Photon Transport in Highly Scattering Tissue
Sigrid Avrillier; Britton Chance; Gerhard J. Mueller; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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