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Proceedings Paper

Time-resolved spectroscopy of mitochondria, cells, and rat tissues under normal and pathological conditions
Author(s): Bertrand Beauvoit; Toshiyuki Kitai; Hanli Liu; Britton Chance
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Paper Abstract

In this study, the detailed dependence of the light scattering on the tissue architecture and intracellular composition was investigated. The reduced scattering coefficient ((mu) s') of isolated rat liver mitochondria, isolated liver cells and various rat tissues was measured at 780 nm by using time-resolved spectroscopy and a sample-substitution protocol. In a first part, extrapolations of the in vitro data to the in vivo situation showed that the mitochondrial compartment contributes for 73% of the scattering of the hepatocytes and about 100% of that of the whole liver. Finally, by analyzing different normal rat tissues and tumors, we have shown that the tissue (mu) s' is independent on the cell concentration in the tissue but is roughly proportional to the tissue mitochondrial content.

Paper Details

Date Published: 31 January 1995
PDF: 10 pages
Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); doi: 10.1117/12.200813
Show Author Affiliations
Bertrand Beauvoit, Univ. of Pennsylvania (United States)
Toshiyuki Kitai, Univ. of Pennsylvania (United States)
Hanli Liu, Univ. of Pennsylvania (United States)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 2326:
Photon Transport in Highly Scattering Tissue
Sigrid Avrillier; Britton Chance; Gerhard J. Mueller; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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