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Proceedings Paper

Particle position measuring with optical tweezers using video processing
Author(s): Sławomir Drobczyński; Marcin Bacia; Marta Woźniak; Krzysztof Symonowicz
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Paper Abstract

The stiffness of a single optical trap is a basic parameter of optical manipulators and detecting the displacement of trapped object belongs to fundamental measurements [1]. The trap stiffness can be determined by position measurements of oscillating (with high frequency) dielectric microsphere. Usually the signal is captured by simple detector like quadrant photodiode [2]. However, this method is hard to apply when working in multitrap mode, especially in case of holographic optical tweezers. In multitrap mode, widely used method is video image processing focused on determining the center of mass of an object [3]. In this contribution we present the method for position measurment based on video sequence analysis from fast camera. The position is obtained by correlating the video frame with templates of known positions of that object. This algorithm can work on several objects, finding their positions independently. It has also scalable accuracy up to sub-pixel level.

Paper Details

Date Published: 18 December 2012
PDF: 6 pages
Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970X (18 December 2012); doi: 10.1117/12.2007276
Show Author Affiliations
Sławomir Drobczyński, Wroclaw Univ. of Technology (Poland)
Marcin Bacia, Wroclaw Univ. of Technology (Poland)
Marta Woźniak, Wrocław Medical Univ. (Poland)
Krzysztof Symonowicz, Wrocław Medical Univ. (Poland)

Published in SPIE Proceedings Vol. 8697:
18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Jan Peřina Jr.; Libor Nozka; Miroslav Hrabovský; Dagmar Senderáková; Waclaw Urbańczyk; Ondrej Haderka; Libor Nožka, Editor(s)

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