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Proceedings Paper

Quantitative phase contrast microscopy in turbid microfluidic channels by digital holography
Author(s): Vittorio Bianco; Melania Paturzo; Andrea Finizio; Pasquale Memmolo; Roberto Puglisi; Donatella Balduzzi; Andrea Galli; Pietro Ferraro
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Paper Abstract

Clear coherent imaging through turbid media is a challenging task showing potentialities in the newest applications in microfluidics. If the targets of interest are dipped into turbid fluids, the medium particles act as strong scatterers, resulting in speckle noise and hindering a clear vision by conventional Optics. Conversely, Digital Holography is able to overcome this limit in case of both flowing and quasi-static media. If the liquid flows at sufficient speed into the microfluidic channel, the Doppler effect can be exploited to record the only useful information. In the quasistatic case, a method is proposed to reduce the speckle noise by processing multiple holograms. Experiments have been carried out to show that a clear amplitude and phase-contrast mapping is achievable by speckle reduction while preserving the image resolution.

Paper Details

Date Published: 11 September 2012
PDF: 6 pages
Proc. SPIE 8413, Speckle 2012: V International Conference on Speckle Metrology, 84131V (11 September 2012); doi: 10.1117/12.2007273
Show Author Affiliations
Vittorio Bianco, Istituto Nazionale di Ottica (Italy)
Melania Paturzo, Istituto Nazionale di Ottica (Italy)
Andrea Finizio, Istituto Nazionale di Ottica (Italy)
Pasquale Memmolo, Istituto Nazionale di Ottica (Italy)
Instituto Italiano di Tecnologia (Italy)
Roberto Puglisi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
Donatella Balduzzi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
Andrea Galli, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
Pietro Ferraro, Istituto Nazionale di Ottica (Italy)

Published in SPIE Proceedings Vol. 8413:
Speckle 2012: V International Conference on Speckle Metrology
Ángel Fernandez Doval; Cristina Trillo, Editor(s)

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