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Proceedings Paper

Towards microscopic resolution in holoscopy
Author(s): Gesa Lilith Franke; Dierck Hillmann; Christian Lührs; Peter Koch; Jörn Wollenzin; Gereon Hüttmann
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Paper Abstract

Holoscopy is a new imaging approach combining digital holography and full-field Fourier-domain optical coherence tomography. The interference pattern between light scattered by a sample and a defined reference wave is recorded and processed numerically. During reconstruction numerical refocusing is applied, overcoming the limitation of the focal depth and thus a uniform, diffraction limited lateral resolution over the whole measurement depth can be obtained. The advantage of numerical refocusing becomes especially significant for imaging at high numerical apertures (NAs). We use a high-resolution setup based on a Mach-Zehnder interferometer with an high-resolution microscope objective (NA = 0.75). For reliable reconstruction of a sample volume the Rayleigh length of the microscope objective and the axial resolution, given by the spectral range of the light source, need to be matched. For a 0.75 NA objective a tunable light source with a sweeping range of ! 300nm is required. Here we present as a first step a tunable Ti:sapphire laser with a tuning range of 187 nm. By characterizing the spectral properties of the Ti:sapphire laser and determining the axial point spread function we demonstrate the feasibility of this light source for high-resolution holoscopy.

Paper Details

Date Published: 20 March 2013
PDF: 6 pages
Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 85711O (20 March 2013); doi: 10.1117/12.2006806
Show Author Affiliations
Gesa Lilith Franke, Univ. zu Lübeck (Germany)
Medical Laser Ctr. Lübeck (Germany)
Dierck Hillmann, Thorlabs GmbH (Germany)
Christian Lührs, Thorlabs GmbH (Germany)
Peter Koch, Thorlabs GmbH (Germany)
Jörn Wollenzin, Thorlabs GmbH (Germany)
Gereon Hüttmann, Univ. zu Lübeck (Germany)

Published in SPIE Proceedings Vol. 8571:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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