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Proceedings Paper

High sensitive fundus autofluorescence imaging combined with speckle-free optical coherence tomography
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Paper Abstract

Scattering and fluorescence images provide complementary information about the health condition of the human eye, so getting them in a single measurement, using a single device may significantly improve a quality of diagnosis as it has been already demonstrated in Spectralis (Heidelberg Eng.) OCT instrument. There is still challenge to improve quality of fundus autofluorescence (FAF) images. The biggest obstacle in obtaining in vivo images of sufficient quality is very low fluorescence signal. For eye safety reasons, and because of patient comfort, using highpower fluorescence excitation is not an adequate solution to the low signal problem. In this contribution we show a new detection method in the retinal autofluorescence imaging, which may improve the sensitivity. We used a fast modulated (up to 500 MHz) diode laser of wavelength 473 nm and detected fluorescence in the spectral range 500-680 nm by photomultiplier and lock-in amplifier. Average power of the collimated blue beam on the cornea used for FAF measurements was set to 50 μW, 10 μW, and even 4.5 μW.

Paper Details

Date Published: 20 March 2013
PDF: 5 pages
Proc. SPIE 8571, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII, 857119 (20 March 2013); doi: 10.1117/12.2006729
Show Author Affiliations
Patrycjusz Stremplewski, Nicolaus Copernicus Univ. (Poland)
Katarzyna Komar, Nicolaus Copernicus Univ. (Poland)
Maciej Szkulmowski, Nicolaus Copernicus Univ. (Poland)
Marta Motoczyńska, Nicolaus Copernicus Univ. (Poland)
Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)

Published in SPIE Proceedings Vol. 8571:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVII
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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