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Proceedings Paper

Needle endomicroscope with a plastic, achromatic objective to perform optical biopsies of breast tissue
Author(s): Matthew Kyrish; Jessica Dobbs; Rebecca Richards-Kortum; Tomasz Tkaczyk
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Paper Abstract

In order to diagnose cancer in breast tissue, a sample must be removed, prepared, and examined under a microscope. To provide an alternative to conventional biopsies, an endomicroscope intended to perform optical biopsies is demonstrated. The system provides high resolution, high contrast images in real-time which could allow a diagnosis to be made during surgery without the need for tissue removal. Optical sectioning is achieved via structured illumination to reject out of focus light. An image is relayed between the sample plane and the imaging system by a coherent fiber bundle with an achromatized objective lens at the distal tip of the fiber bundle which is the diameter of a biopsy needle. The custom, plastic objective provides correction for both the excitation and emission wavelengths of proflavine (452 nm and 515 nm, respectively). It also magnifies the object onto the distal tip of the fiber bundle to increase lateral resolution. The lenses are composed of the optical plastics Zeonex E48R, PMMA, and polystyrene. The lenses are fabricated via single point diamond turning and assembled using a zero alignment technique. The lateral resolution and chromatic focal shift were measured and in vitro images of breast carcinoma cells stained with proflavine were captured. The optical biopsy system is able to achieve optical sectioning and to resolve smaller features than the current high resolution microendoscope.

Paper Details

Date Published: 13 March 2013
PDF: 9 pages
Proc. SPIE 8575, Endoscopic Microscopy VIII, 857505 (13 March 2013); doi: 10.1117/12.2005219
Show Author Affiliations
Matthew Kyrish, Rice Univ. (United States)
Jessica Dobbs, Rice Univ. (United States)
Rebecca Richards-Kortum, Rice Univ. (United States)
Tomasz Tkaczyk, Rice Univ. (United States)

Published in SPIE Proceedings Vol. 8575:
Endoscopic Microscopy VIII
Guillermo J. Tearney M.D.; Thomas Duen-Shyr Wang, Editor(s)

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