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Proceedings Paper

An overview of time-domain diffuse fluorescence imaging: instrumentation and applications
Author(s): Kenneth M. Tichauer; Robert W. Holt; Frederic Leblond; Brian W. Pogue
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Paper Abstract

Rapidly pulsed laser excitation with high-rate signal detection allows the dispersion of emitted fluorescent signals through tissue to be measured. This “time-domain” approach to measuring diffuse fluorescence data offers a wide range of advantages over conventional “continuous-wave” fluorescence imaging. While continuous-wave imaging provides a measure of only fluorescence intensity at each source-detector pairing, time-domain approaches provide fluorescence intensity throughout the whole temporal response to the pulsed-laser excitation, adding a whole new dimension to the dataset. This paper introduces the instrumentation required to collect time-domain data, dealing with complications and nuances of system and data calibration, as well as the major current applications of time-domain diffuse fluorescence imaging.

Paper Details

Date Published: 25 March 2013
PDF: 9 pages
Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 857828 (25 March 2013); doi: 10.1117/12.2005188
Show Author Affiliations
Kenneth M. Tichauer, Illinois Institute of Technology (United States)
Thayer School of Engineering at Dartmouth (United States)
Robert W. Holt, Dartmouth College (United States)
Frederic Leblond, Thayer School of Engineering at Dartmouth (United States)
Ecole Polytechnique de Montréal (Canada)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)
Dartmouth College (United States)

Published in SPIE Proceedings Vol. 8578:
Optical Tomography and Spectroscopy of Tissue X
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca, Editor(s)

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