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Proceedings Paper

Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy
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Paper Abstract

Different techniques have been developed to determine the optical properties of turbid media, which include collimated transmission, diffuse reflectance, adding-doubling and goniometry. While goniometry can be used to determine the anisotropy of scattering (g), other techniques are used to measure the absorption coefficient and reduced scattering coefficient (μs(1-g)). But separating scattering coefficient (μs) and anisotropy of scattering from reduced scattering coefficient has been tricky. We developed an algorithm to determine anisotropy of scattering from the depth dependent decay of reflectance-mode confocal scanning laser microscopy (rCSLM) data. This report presents the testing of the algorithm on tissue phantoms with different anisotropies (g = 0.127 to 0.868, at 488 nm wavelength). Tissue phantoms were made from polystyrene microspheres (6 sizes 0.1-0.5 μm dia.) dispersed in both aqueous solutions and agarose gels. Three dimensional images were captured. The rCSLM-signal followed an exponential decay as a function of depth of the focal volume, R(z)ρexp(-μz) where ρ (dimensionless, ρ = 1 for a mirror) is the local reflectivity and μ [cm-1] is the exponential decay constant. The theory was developed to uniquely map the experimentally determined μ and ρ into the optical scattering properties μs and g. The values of μs and g depend on the composition and microstructure of tissues, and allow characterization of a tissue.

Paper Details

Date Published: 21 February 2013
PDF: 7 pages
Proc. SPIE 8592, Biomedical Applications of Light Scattering VII, 85920U (21 February 2013); doi: 10.1117/12.2005072
Show Author Affiliations
Ravikant Samatham, Oregon Health & Science Univ. (United States)
Steven L. Jacques, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 8592:
Biomedical Applications of Light Scattering VII
Adam P. Wax; Vadim Backman, Editor(s)

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