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Proceedings Paper

GPU-based image registration in aperture correlation microscopy, and reflection mode correlation microscopy
Author(s): Lionel J. Fafchamps; Mark A. A. Neil; Rimas Juskaitis
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Paper Abstract

Aperture Correlation Microscopy (ACM) is a fluorescence microscopy technique capable of depth resolved imaging and enhanced lateral resolution at real-time acquisition rates. It relies on the subtraction of 2 separate images from different cameras which must be registered to the sub-pixel level. In order to achieve real-time registration and subtraction, the graphics processing unit (GPU) is used to apply a transformation from one frame to the other, resulting in a system capable of processing over 200 frames per second on modest hardware (GeForce 330M). Currently, this rate is limited by camera acquision to 16fps. Additionally, a novel reflection mode correlation microscope is introduced which functions on similar principles as the fluorescent system but can be used to examine reflective samples. Images and z-stacks taken with this system are presented here.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858907 (22 February 2013); doi: 10.1117/12.2004968
Show Author Affiliations
Lionel J. Fafchamps, Imperial College London (United Kingdom)
Mark A. A. Neil, Imperial College London (United Kingdom)
Rimas Juskaitis, Aurox Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 8589:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Carol J. Cogswell; Thomas G. Brown; Jose-Angel Conchello; Tony Wilson, Editor(s)

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