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Proceedings Paper

Modification of a commercially available photoacoustic imaging system for the use of 1064nm and 532nm wavelengths to assess photoacoustic contrast agents
Author(s): Andrew Heinmiller; Kimberly Homan; Stanislav Emelianov; Adam Cole; Sanjiv Gambhir; Andrew Needles; Catherine Theodoropoulos; Desmond Hirson
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Paper Abstract

The use of near-infrared wavelengths for photoacoustic (PA) imaging takes advantage of the relatively low inherent absorption of tissues and has encouraged the development of agents which show high contrast in this range. Here, we describe the modification of a commercially available PA imaging system (Vevo LAZR, VisualSonics, Toronto) to take advantage of the 532nm and 1064nm wavelengths inherent in the generation of the currently tuneable range of 680 to 970nm and in the use of these two wavelengths to assess contrast agents. The photoacoustic imaging system generated light from a Nd/YAG laser modified to extract the 532 and 1064nm wavelengths in addition to its OPO-derived tuneable range (680 - 970 nm) and deliver this light through a fiber integrated into a linear array transducer (LZ400, VisualSonics). Gold nanorods (UT Austin), carbon nanotubes (Stanford U), DyLight 550 (Thermo Fisher) and blood were imaged in a phantom (PE20 tubing) and in a hindlimb subcutaneous tumor in vivo to determine their photoacoustic signal intensity at all wavelengths. In the phantom and in vivo, all agents caused an enhancement of the photoacoustic signal at their respective peak absorbance wavelengths. These results show that the 532nm and 1064nm wavelengths could prove useful in biomedical imaging due to the contrast agents customized for them. The 1064nm wavelength in particular has the advantage of having very low generation of endogenous signal in vivo, making agents tuned to this wavelength ideal for targeted contrast imaging.

Paper Details

Date Published: 4 March 2013
PDF: 7 pages
Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85813O (4 March 2013); doi: 10.1117/12.2004804
Show Author Affiliations
Andrew Heinmiller, VisualSonics Inc. (Canada)
Kimberly Homan, Univ. of Texas at Austin (United States)
Stanislav Emelianov, Univ. of Texas at Austin (United States)
Adam Cole, Stanford Univ. (United States)
Sanjiv Gambhir, Stanford Univ. (United States)
Andrew Needles, VisualSonics Inc. (Canada)
Catherine Theodoropoulos, VisualSonics Inc. (Canada)
Desmond Hirson, VisualSonics Inc. (Canada)

Published in SPIE Proceedings Vol. 8581:
Photons Plus Ultrasound: Imaging and Sensing 2013
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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