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Proceedings Paper

In vivo optical activation of astrocytes as a potential therapeutic strategy for neurodegenerative diseases
Author(s): Yuanxin Chen; James Mancuso; Zhen Zhao; Xuping Li; Zhong Xue; Stephen T. C. Wong
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Paper Abstract

Neurovascular dysfunction in many neurodegenerative diseases, such as Alzheimer’s disease (AD), reduces blood flow to affected brain areas and causes neuronal dysfunction and loss. A new optical imaging technique is developed to activate astrocytes in live animal models in order to investigate the increase of local cerebral blood flow as a potential therapeutic strategy for AD. The technique uses fluorescent labeling of vasculature and astrocytes coupled with intravital 2-photon microscopy to visualize the astrocyte-vasculature interactions in live animals. Using femtosecond laser stimulation, calcium uncaging is applied to specifically target and activate astrocytes in vivo with high spatial and temporal resolutions. Intravital 2-photon microscopy imaging was employed to demonstrate that single endfoot optical activation around an arteriole induced a 25% increase in arteriole diameter, which in turn increased cerebral local blood flow in down-stream capillaries. This quantitative result indicates the potential of using optical activation of astrocytes in afflicted brain areas of neurodegeneration to restore normal neurovascular functions.

Paper Details

Date Published: 8 March 2013
PDF: 9 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85655K (8 March 2013); doi: 10.1117/12.2004712
Show Author Affiliations
Yuanxin Chen, Weill Cornell Medical College (United States)
James Mancuso, Weill Cornell Medical College (United States)
Zhen Zhao, Weill Cornell Medical College (United States)
Southeast Univ. Medical School (China)
Xuping Li, Weill Cornell Medical College (United States)
Zhong Xue, Weill Cornell Medical College (United States)
Stephen T. C. Wong, Weill Cornell Medical College (United States)

Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg M.D.; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory M.D.; Guillermo J. Tearney M.D.; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen M.D.; E. Duco Jansen; Justus F. Ilgner M.D.; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

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