Share Email Print

Proceedings Paper

Fluorescence lifetime imaging microscopy of nanodiamonds in vivo
Author(s): Yung Kuo; Tsung-Yuan Hsu; Yi-Chun Wu; Jui-Hung Hsu; Huan-Cheng Chang
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The negatively charged nitrogen-vacancy (NV) center in bulk diamond is a photostable fluorophore with a radiative lifetime of 11.6 ns at room temperature. The lifetime substantially increases to ~20 ns for diamond nanoparticles (size ~ 100 nm) suspended in water due to the change in refractive index of the surrounding medium of the NV centers. This fluorescence decay time is much longer than that (typically 1 − 4 ns) of endogenous and exogenous fluorophores commonly used in biological imaging, making it possible to detect NV-containing nanodiamonds in vivo at the single particle level by fluorescence lifetime imaging microscopy (FLIM). We demonstrate the feasibility of this approach using Caenorhabditis elegans (C. elegans) as a model organism.

Paper Details

Date Published: 29 March 2013
PDF: 7 pages
Proc. SPIE 8635, Advances in Photonics of Quantum Computing, Memory, and Communication VI, 863503 (29 March 2013); doi: 10.1117/12.2004494
Show Author Affiliations
Yung Kuo, Institute of Atomic and Molecular Sciences (Taiwan)
Tsung-Yuan Hsu, National Taiwan Univ. (Taiwan)
Yi-Chun Wu, National Taiwan Univ. (Taiwan)
Jui-Hung Hsu, National Sun Yat-Sen Univ. (Taiwan)
Huan-Cheng Chang, Institute of Atomic and Molecular Sciences (Taiwan)

Published in SPIE Proceedings Vol. 8635:
Advances in Photonics of Quantum Computing, Memory, and Communication VI
Zameer U. Hasan; Philip R. Hemmer; Hwang Lee; Charles M. Santori, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?