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Proceedings Paper

Single-molecule imaging can be achieved in live obligate anaerobic bacteria
Author(s): Krishanthi S. Karunatilaka; Ben R. Coupland; Elizabeth A. Cameron; Eric C. Martens; Nicole K. Koropatkin; Julie S. Biteen
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Paper Abstract

Single-molecule fluorescence (SMF) permits imaging with nanometer-scale resolution. This technique is particularly useful for cellular imaging as it provides a non-invasive, minimally perturbative means to examine macromolecular localization and dynamics, even in live cells. Here, we demonstrate that nanometer-scale SMF imaging can be extended to a new category of experiments: intracellular imaging of live, obligate anaerobic cells on the benchtop. We investigate the starch-utilization system (Sus) proteins in the gut symbiont Bacteroides thetaiotaomicron and discuss three different labels that we implemented to detect these proteins: fluorescent proteins, the tetracysteine-based FlAsH tag, and the enzymatic HaloTag.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900K (22 February 2013); doi: 10.1117/12.2004204
Show Author Affiliations
Krishanthi S. Karunatilaka, Univ. of Michigan (United States)
Ben R. Coupland, Univ. of Michigan (United States)
Elizabeth A. Cameron, Univ. of Michigan Medical School (United States)
Eric C. Martens, Univ. of Michigan Medical School (United States)
Nicole K. Koropatkin, Univ. of Michigan Medical School (United States)
Julie S. Biteen, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8590:
Single Molecule Spectroscopy and Superresolution Imaging VI
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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