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Proceedings Paper

IFSA: a microfluidic chip-platform for frit-based immunoassay protocols
Author(s): Nadine Hlawatsch; Michael Bangert; Peter Miethe; Holger Becker; Claudia Gärtner
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Paper Abstract

Point-of-care diagnostics (POC) is one of the key application fields for lab-on-a-chip devices. While in recent years much of the work has concentrated on integrating complex molecular diagnostic assays onto a microfluidic device, there is a need to also put comparatively simple immunoassay-type protocols on a microfluidic platform. In this paper, we present the development of a microfluidic cartridge using an immunofiltration approach. In this method, the sandwich immunoassay takes place in a porous frit on which the antibodies have immobilized. The device is designed to be able to handle three samples in parallel and up to four analytical targets per sample. In order to meet the critical cost targets for the diagnostic market, the microfluidic chip has been designed and manufactured using high-volume manufacturing technologies in mind. Validation experiments show comparable sensitivities in comparison with conventional immunofiltration kits.

Paper Details

Date Published: 13 March 2013
PDF: 5 pages
Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150J (13 March 2013); doi: 10.1117/12.2004127
Show Author Affiliations
Nadine Hlawatsch, microfluidic ChipShop GmbH (Germany)
Michael Bangert, Research Ctr. of Medical Technology and Biotechnology (Germany)
Peter Miethe, Research Ctr. of Medical Technology and Biotechnology (Germany)
Holger Becker, microfluidic ChipShop GmbH (Germany)
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 8615:
Microfluidics, BioMEMS, and Medical Microsystems XI
Holger Becker; Bonnie L. Gray, Editor(s)

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