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Proceedings Paper

Single shot white light interference microscopy with colour fringe analysis for quantitative phase imaging of biological cells
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Paper Abstract

To quantitatively obtain the phase map of Onion and human red blood cell (RBC) from white light interferogram we used Hilbert transform color fringe analysis technique. The three Red, Blue and Green color components are decomposed from single white light interferogram and Refractive index profile for Red, Blue and Green colour were computed in a completely non-invasive manner for Onion and human RBC. The present technique might be useful for non-invasive determination of the refractive index variation within cells and tissues and morphological features of sample with ease of operation and low cost.

Paper Details

Date Published: 22 February 2013
PDF: 5 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858706 (22 February 2013); doi: 10.1117/12.2003994
Show Author Affiliations
Vishal Srivastava, India Institute of Technology Dehli (India)
D. S. Mehta, Indian Institute of Technology Delhi (India)

Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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