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Proceedings Paper

In vivo imaging of collagen fiber orientation with rapid polarization-resolved SHG microscopy
Author(s): Yuji Tanaka; Eiji Hase; Shuichiro Fukushima; Takeshi Yasui; Tsutomu Araki
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Paper Abstract

Polarization-resolved second-harmonic-generation (SHG) microscopy is a powerful tool to visualize distribution of collagen fiber orientation in tissue with little invasion. However, long image acquisition time, resulting from mechanical rotation of a half-wave plate, makes this microscopy easy to suffer from motion artifact of a sample and hence has limited its use to in vivo application. In this paper, we constructed rapid, polarization-resolved SHG microscopy by combination of an electro-optics-modulator-based polarization modulation with improved data acquisition method. The constructed polarization-resolved SHG microscopy enables us to visualize orientation mapping of dermal collagen fiber in rat skin and human one in vivo without influence of motion artifact. This microscope will open the door for in vivo measurement of collagen fiber orientation in human skin.

Paper Details

Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882X (22 February 2013); doi: 10.1117/12.2003969
Show Author Affiliations
Yuji Tanaka, Osaka Univ. (Japan)
Eiji Hase, Univ. of Tokushima (Japan)
Shuichiro Fukushima, Osaka Univ. (Japan)
Takeshi Yasui, Osaka Univ. (Japan)
Univ. of Tokushima (Japan)
Tsutomu Araki, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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