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Proceedings Paper

Imaging of the mouse lung with scanning laser optical tomography (SLOT)
Author(s): Marko Heidrich; Manuela Kellner; Rebecca Beigel; Raoul-Amadeus Lorbeer; Lars Knudsen; Alexander Heisterkamp; Tammo Ripken; Matthias Ochs; Mark Philipp Kühnel; Heiko Meyer
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Paper Abstract

New optical techniques have the potential to fill the gap between radiological and microscopic approaches to assess the lung's internal structure. Since its quantitative assessment requires unbiased sampling and measurement principles, imaging of the whole lung with sufficient resolution for visualizing details is important. To address this request, we applied scanning laser optical tomography (SLOT) for the three dimensional imaging of mouse lung ex vivo. SLOT is a highly efficient flourescence and transmission microscopy technique allowing for 3D imaging of specimen of sizes up to several millimeters. Previously fixed lung lobes and whole lungs were optically cleared and subsequently imaged with SLOT while making use of intrinsic contrast mechanisms like absorption and autofluorescence. Imaging of airways, blood vessels and parenchyma is demonstrated. Volumetric SLOT datasets of the lung's internal structure can be analyzed in any preferred planar orientation. Moreover, the sample preparation preserves microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, SLOT is a useful technique to visualize and survey the internal structure of mouse lung at different scales and with various contrast mechanisms. Potential applications of SLOT in lung research are e.g. quantitative phenotype analysis of mouse models of human lung disease in combination with stereological methods.

Paper Details

Date Published: 8 March 2013
PDF: 8 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 856535 (8 March 2013); doi: 10.1117/12.2003955
Show Author Affiliations
Marko Heidrich, Laser Zentrum Hannover e.V. (Germany)
Manuela Kellner, Hannover Medical School (Germany)
Rebecca Beigel, Hannover Medical School (Germany)
REBIRTH Cluster of Excellence (Germany)
Raoul-Amadeus Lorbeer, Laser Zentrum Hannover e.V. (Germany)
Lars Knudsen, Hannover Medical School (Germany)
Member of the German Ctr. for Lung Research (Germany)
Alexander Heisterkamp, REBIRTH Cluster of Excellence (Germany)
Member of the German Ctr. for Lung Research (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Tammo Ripken, Laser Zentrum Hannover e.V. (Germany)
REBIRTH Cluster of Excellence (Germany)
Matthias Ochs, Hannover Medical School (Germany)
REBIRTH Cluster of Excellence (Germany)
Member of the German Ctr. for Lung Research (Germany)
Mark Philipp Kühnel, Hannover Medical School (Germany)
Heiko Meyer, Laser Zentrum Hannover e.V. (Germany)
REBIRTH Cluster of Excellence (Germany)
Hannover Medical School (Germany)

Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg M.D.; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory M.D.; Guillermo J. Tearney M.D.; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen M.D.; E. Duco Jansen; Justus F. Ilgner M.D.; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

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