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Proceedings Paper

Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy
Author(s): Nirmal Mazumder; Jianjun Qiu; Chih-Wei Hu; Fu-Jen Kao
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Paper Abstract

We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858823 (22 February 2013); doi: 10.1117/12.2003894
Show Author Affiliations
Nirmal Mazumder, National Yang-Ming Univ. (Taiwan)
Jianjun Qiu, National Yang-Ming Univ. (Taiwan)
Chih-Wei Hu, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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